DEDD2 Polyclonal antibody

DEDD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14574-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

DED containing protein FLAME 3, DED-containing protein FLAME-3, DNA-binding death effector domain-containing protein 2, FADD-like anti-apoptotic molecule 3, FLAME 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14574-1-AP targets DEDD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag5724

Product name: Recombinant human DEDD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-326 aa of BC027930

Sequence: MALSGSTPAPCWEEDECLDYYGMLSLHRMFEVVGGQLTECELELLAFLLDEAPGAAGGLARARSGLELLLELERRGQCDESNLRLLGQLLRVLARHDLLPHLARKRRRPVSPERYSYGTSSSSKRTEGSCRRRRQSSSSANSQQGQWETGSPPTKRQRRSRGRPSGGARRRRRGAPAAPQQQSEPARPSSEGKVTCDIRLRVRAEYCEHGPALEQGVASRRPQALARQLDVFGQATAVLRSRDLGSVVCDIKFSELSYLDAFWGDYLSGALLQALRGVFLTEALREAVGREAVRLLVSVDEADYEAGRRRLLLMEEEGGRRPTEAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 death effector domain containing 2
别名 DED containing protein FLAME 3, DED-containing protein FLAME-3, DNA-binding death effector domain-containing protein 2, FADD-like anti-apoptotic molecule 3, FLAME 3
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC027930
基因名称 DEDD2
Gene ID (NCBI) 162989
RRIDAB_2091538
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXF8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEDD2 (Death Effector Domain-containing protein 2) is a conserved pro-apoptotic protein that harbors a single death effector domain and is predominantly expressed in liver, kidney and leukocytes. Upon overexpression DEDD2 translocates to the nucleus via a bipartite nuclear localization signal, interacts with caspase-8/10 and sensitizes cells to Fas- or TRAIL-induced apoptosis, but not to Bax- or staurosporine-mediated death . Its nuclear import is essential for apoptotic activity, positioning DEDD2 as a regulator of death-receptor-triggered nuclear events (PMID: 11741985).

实验方案

Product Specific Protocols
WB protocol for DEDD2 antibody 14574-1-APDownload protocol
IHC protocol for DEDD2 antibody 14574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Differ

Coordinated repression of BIM and PUMA by Epstein-Barr virus latent genes maintains the survival of Burkitt lymphoma cells.

Authors - Leah Fitzsimmons
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