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验证数据展示

  • IHC staining of mouse lung using 25855-1-AP

    IHC staining of mouse lung using 25855-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25855-1-AP (DECR2 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of mouse lung using 25855-1-AP

    IHC staining of mouse lung using 25855-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25855-1-AP (DECR2 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of mouse lung using 25855-1-AP

    WB analysis of mouse lung using 25855-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25855-1-AP (DECR2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

DECR2 Polyclonal antibody

DECR2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25855-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

2,4 dienoyl CoA reductase 2, DECR2, PDCR, SDR17C1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25855-1-AP targets DECR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag23052

Product name: Recombinant human DECR2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-244 aa of BC011968

Sequence: MRHGCHTVIASRSLPRVLTAARKLAGATGRRCLPLSMDVRAPPAVMAAVDQALKEFGRIDILINCAAGNFLCPAGALSFNAFKTVMDIDTSGTFNVSRVLYEKFFRDHGGVIVNITATLGNRGQALQVHAGSAKAAVDAMTRHLAVEWGPQNIRVNSLAPGPISGTEGLRRLGGPQASLSTKVTASPLQRLGNKTEIAHSVLYLASPLASYVTGAVLVADGGAWLTFPNGVKGLPDFASFSAKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 2,4-dienoyl CoA reductase 2, peroxisomal
别名 2,4 dienoyl CoA reductase 2, DECR2, PDCR, SDR17C1
计算分子量 292 aa, 31 kDa
观测分子量 30 kDa
GenBank蛋白编号BC011968
基因名称 DECR2
Gene ID (NCBI) 26063
RRIDAB_2880270
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUI1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DECR2 antibody 25855-1-APDownload protocol
IHC protocol for DECR2 antibody 25855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

The p97-UBXD8 complex maintains peroxisome abundance by suppressing pexophagy

Authors - Iris D Montes
View Article