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验证数据展示

  • IF Staining of human stomach using 82969-4-RR (same clone as 82969-4-PBS)

    IF Staining of human stomach using 82969-4-RR (same clone as 82969-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach tissue using DDX60 antibody (82969-4-RR, Clone: 230293G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82969-4-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82969-4-RR (same clone as 82969-4-PBS)

    FC experiment of A431 using 82969-4-RR (same clone as 82969-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human DDX60 (82969-4-RR, Clone:230293G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82969-4-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 82969-4-RR (same clone as 82969-4-PBS)

    IHC staining of human stomach using 82969-4-RR (same clone as 82969-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82969-4-RR (DDX60 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82969-4-PBS in a different storage buffer formulation.

DDX60 Recombinant monoclonal antibody, PBS Only

DDX60 Uni-rAb® Recombinant Antibody for IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 82969-4-PBS

产品说明书

CloneNo. 230293G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF-P, FC (Intra), Indirect ELISA

别名

230293G10, DEAD box protein 60, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX60

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82969-4-PBS targets DDX60 in IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34406

Product name: Recombinant human DDX60 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1135-1250 aa of BC038115

Sequence: KLGAVENAAESVSTFLKKKQETKRPPKADKEAHVMANKLRKVKKSIEKQKIIDEKSQKKTRNVDQSLIHEAEHDNLVKCLEKNLEIPQDCTYADQKAVDTETLQKVFGRVKFERKG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
别名 230293G10, DEAD box protein 60, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX60
计算分子量 1712 aa, 198 kDa
观测分子量 170-200 kDa
GenBank蛋白编号BC038115
基因名称 DDX60
Gene ID (NCBI) 55601
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IY21
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.

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