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DDX54 Polyclonal antibody

DDX54 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26894-1-AP

Print datasheet

Synonyms

DDX54, DEAD box protein 54, DEAD box RNA helicase 97 kDa, DP97

验证数据展示

  • WB analysis of HeLa using 26894-1-AP

    WB analysis of HeLa using 26894-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26894-1-AP (DDX54 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 26894-1-AP

    IF Staining of PC-3 using 26894-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DDX54 antibody (26894-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26894-1-AP targets DDX54 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen DDX54 fusion protein Ag25289 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Synonyms DDX54, DEAD box protein 54, DEAD box RNA helicase 97 kDa, DP97
Calculated Molecular Weight 98 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC156669
Gene Symbol DDX54
Gene ID (NCBI) 79039
RRIDAB_2880671
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDD1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX54, also named as ATP-dependent RNA helicase DDX54, is a 881 amino acid protein, which contains 1 helicase ATP-binding domain and belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. DDX54 localizes in nucleus and Interacts in a hormone-dependent manner with nuclear receptors. DDX54 has RNA-dependent ATPase activity and represses the transcriptional activity of nuclear receptors.

实验方案

Product Specific Protocols
WB protocol for DDX54 antibody 26894-1-APDownload protocol
IF protocol for DDX54 antibody 26894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

Nucleolar dynamics are determined by the ordered assembly of the ribosome

Authors - Jessica Sheu-Gruttadauria
  • KD Validated
View Article