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验证数据展示

  • WB analysis using 88502-1-RR (same clone as 88502-1-PBS)

    WB analysis using 88502-1-RR (same clone as 88502-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88502-1-RR (DDX5,p68 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88502-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 88502-1-RR (same clone as 88502-1-PBS)

    IF Staining of HepG2 using 88502-1-RR (same clone as 88502-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5,p68 antibody (88502-1-RR, Clone: 252934E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 88502-1-PBS in a different storage buffer formulation.

DDX5,p68 Recombinant monoclonal antibody, PBS Only

DDX5,p68 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 88502-1-PBS

产品说明书

CloneNo. 252934E11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

DDX5, EC:3.6.4.13, G17P1, HELR, HLR1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DDX5 Ready-To-Use IHC Kit. DDX5 primary antibody included.

产品信息

88502-1-PBS targets DDX5,p68 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag40657

Product name: Recombinant human DDX5,p68 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 265-614 aa of BC016027

Sequence: IVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAAAPMIGYPMPTGYSQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
别名 DDX5, EC:3.6.4.13, G17P1, HELR, HLR1
计算分子量 69 kDa
观测分子量 65-68 kDa
GenBank蛋白编号BC016027
基因名称 DDX5
Gene ID (NCBI) 1655
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP17844
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation.

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