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Species Reactivity
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Species Reactivity
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验证数据展示

  • WB analysis using 67025-1-Ig (same clone as 67025-1-PBS)

    WB analysis using 67025-1-Ig (same clone as 67025-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 67025-1-Ig (same clone as 67025-1-PBS)

    IP experiment of HeLa using 67025-1-Ig (same clone as 67025-1-PBS)

    IP result of anti-DDX5 (IP:67025-1-Ig, 5ug; Detection:67025-1-Ig 1:1000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67025-1-Ig (same clone as 67025-1-PBS)

    IHC staining of human colon cancer using 67025-1-Ig (same clone as 67025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67025-1-Ig (DDX5 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX5 antibody included
  • IF Staining of HepG2 using 67025-1-Ig (same clone as 67025-1-PBS)

    IF Staining of HepG2 using 67025-1-Ig (same clone as 67025-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (67025-1-Ig, Clone: 2D3D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

  • WB analysis using 67025-1-Ig (same clone as 67025-1-PBS)

    WB analysis using 67025-1-Ig (same clone as 67025-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67025-1-Ig (DDX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67025-1-PBS in a different storage buffer formulation.

DDX5 Monoclonal antibody, PBS Only

DDX5 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 67025-1-PBS

产品说明书

CloneNo. 2D3D2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

2D3D2, DDX5,p68, EC:3.6.4.13, G17P1, HELR

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DDX5 Ready-To-Use IHC Kit. DDX5 primary antibody included.

产品信息

67025-1-PBS targets DDX5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DDX5 fusion protein Ag24821 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
别名 2D3D2, DDX5,p68, EC:3.6.4.13, G17P1, HELR
观测分子量 69 kDa
GenBank蛋白编号NM_004396
基因名称 DDX5
Gene ID (NCBI) 1655
RRIDAB_2882340
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP17844
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation.