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DDX5 Polyclonal antibody

DDX5 Polyclonal Antibody for WB, IP, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 26385-1-AP

Print datasheet

Synonyms

DDX5, DEAD box protein 5, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68

验证数据展示

  • IP experiment of HeLa using 26385-1-AP

    IP experiment of HeLa using 26385-1-AP

    IP result of anti-DDX5 (IP:26385-1-AP, 4ug; Detection:26385-1-AP 1:500) with HeLa cells lysate 1600 ug.

  • WB analysis of HepG2 using 26385-1-AP

    WB analysis of HepG2 using 26385-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26385-1-AP (DDX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26385-1-AP

    IF Staining of HepG2 using 26385-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (26385-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DDX5 Ready-To-Use IHC Kit. DDX5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26385-1-AP targets DDX5 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IF/ICC, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen DDX5 fusion protein Ag24821 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Synonyms DDX5, DEAD box protein 5, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68
Observed Molecular Weight 69 kDa
GenBank Accession NumberNM_004396
Gene Symbol DDX5
Gene ID (NCBI) 1655
RRIDAB_2880498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17844
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DDX5 antibody 26385-1-APDownload protocol
IF protocol for DDX5 antibody 26385-1-APDownload protocol
IP protocol for DDX5 antibody 26385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Eur J Med Chem

Discovery of potent thiazolidin-4-one sulfone derivatives for inhibition of proliferation of osteosarcoma in vitro and in vivo

Authors - Xuwen Chen
View Article