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验证数据展示

  • WB analysis using 83287-3-RR (same clone as 83287-3-PBS)

    WB analysis using 83287-3-RR (same clone as 83287-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83287-3-RR (DDX47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 83287-3-RR (same clone as 83287-3-PBS)

    WB analysis of HeLa using 83287-3-RR (same clone as 83287-3-PBS)

    WB result of DDX47 antibody (83287-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX47 transfected HeLa cells. This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

    FC experiment of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug DDX47 Recombinant antibody (83287-3-RR, Clone:240193C4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

    IF Staining of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (83287-3-RR, Clone: 240193C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

    IF Staining of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (83287-3-RR, Clone: 240193C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

DDX47 Recombinant monoclonal antibody, PBS Only

DDX47 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83287-3-PBS

产品说明书

CloneNo. 240193C4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

240193C4, DEAD box protein 47, E4 DBP, EC:3.6.4.13, HQ0256

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83287-3-PBS targets DDX47 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11953

Product name: Recombinant human DDX47 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 202-455 aa of BC068009

Sequence: FLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQSKRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRSGKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
别名 240193C4, DEAD box protein 47, E4 DBP, EC:3.6.4.13, HQ0256
计算分子量 455 aa, 51 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC068009
基因名称 DDX47
Gene ID (NCBI) 51202
RRIDAB_3670953
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H0S4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DEAD-box helicase 47 (DDX47) is a member of the DEAD-box protein family. It contains two conserved domains: DEXDc (DEAD-like helicases superfamily) and HELICc (helicase superfamily c-terminal domain) (PMID: 16337753). DDX47 can shuttle between the nucleus and the cytoplasm. In different cancers, evidence has shown that DDX47 is a poor prognostic predictor for renal cell carcinoma, as well as a diagnostic biomarker for breast cancer and chronic myeloid leukemia (PMID: 35490652).

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