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验证数据展示

  • WB analysis using 31211-1-AP (same clone as 31211-1-PBS)

    WB analysis using 31211-1-AP (same clone as 31211-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31211-1-AP (DDX42 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31211-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 31211-1-AP (same clone as 31211-1-PBS)

    IHC staining of mouse testis using 31211-1-AP (same clone as 31211-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31211-1-AP (DDX42 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31211-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 31211-1-AP (same clone as 31211-1-PBS)

    IF Staining of HeLa using 31211-1-AP (same clone as 31211-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX42 antibody (31211-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31211-1-PBS in a different storage buffer formulation.

DDX42 Polyclonal antibody, PBS Only

DDX42 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31211-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

RNA helicase related protein, RNA helicase like protein, RHELP, EC:3.6.4.13, DEAD box protein 42

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31211-1-PBS targets DDX42 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34762

Product name: Recombinant human DDX42 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 456-819 aa of BC015505

Sequence: VARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
别名 RNA helicase related protein, RNA helicase like protein, RHELP, EC:3.6.4.13, DEAD box protein 42
计算分子量 103 kDa
观测分子量130 kDa
GenBank蛋白编号BC015505
基因名称 DDX42
Gene ID (NCBI) 11325
RRIDAB_3669903
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86XP3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX42 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 42) is a member of the DEAD box RNA helicase superfamily. A C-terminal portion of DDX42 interacts with the pro-apoptotic factor, apoptosis-stimulating protein of p53 protein 2 (ASPP2), thereby inhibiting its action (PMID: 30834153).

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