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验证数据展示

  • WB analysis of HeLa using 17087-1-AP

    WB analysis of HeLa using 17087-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 17087-1-AP

    IF Staining of HEK-293 using 17087-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DDX27 antibody (17087-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

DDX27 Polyclonal antibody

DDX27 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17087-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Probable ATP-dependent RNA helicase DDX27, PP3241, HSPC259, EC:3.6.4.13, DEAD box protein 27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, NIH/3T3 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

17087-1-AP targets DDX27 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 DDX27 fusion protein Ag9426 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
别名 Probable ATP-dependent RNA helicase DDX27, PP3241, HSPC259, EC:3.6.4.13, DEAD box protein 27
计算分子量 796 aa, 90 kDa
观测分子量 90 kDa
GenBank蛋白编号BC016060
基因名称 DDX27
Gene ID (NCBI) 55661
RRIDAB_2092569
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96GQ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX27 is one of the DEAD box proteins, which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DEAD box proteins are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division, and DDX27 is involved in the processing of 5.8S and 28S ribosomal RNAs.

实验方案

Product Specific Protocols
WB protocol for DDX27 antibody 17087-1-APDownload protocol
IF protocol for DDX27 antibody 17087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity.

Authors - Francesca Rossi
View Article
humanWB

Onco Targets Ther

DEAD-box helicase 27 plays a tumor-promoter role by regulating the stem cell-like activity of human colorectal cancer cells.

Authors - Chunxing Yang
View Article