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验证数据展示

  • WB analysis using 84103-6-RR (same clone as 84103-6-PBS)

    WB analysis using 84103-6-RR (same clone as 84103-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84103-6-RR (DDX24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84103-6-PBS in a different storage buffer formulation.

  • FC experiment of U-2 OS using 84103-6-RR (same clone as 84103-6-PBS)

    FC experiment of U-2 OS using 84103-6-RR (same clone as 84103-6-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug DDX24 Recombinant antibody (84103-6-RR, Clone:241145G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84103-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84103-6-RR (same clone as 84103-6-PBS)

    IF Staining of HeLa using 84103-6-RR (same clone as 84103-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX24 antibody (84103-6-RR, Clone: 241145G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84103-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84103-6-RR

    Affinity and Kinetic Characterization of 84103-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84103-6-RR against Human DDX24 were performed. The affinity constant is below 1 pM.

DDX24 Recombinant monoclonal antibody, PBS Only

DDX24 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84103-6-PBS

产品说明书

CloneNo. 241145G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

EC:3.6.4.13, DDX 24, ATP-dependent RNA helicase DDX24, 241145G10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84103-6-PBS targets DDX24 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8407

Product name: Recombinant human DDX24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 509-859 aa of BC008847

Sequence: TLTLVHQAPARILHKKHTKKMDKTAKLDLLMQKIGMRGKPKVIDLTRNEATVETLTETKIHCETDEKDFYLYYFLMQYPGRSLVFANSISCIKRLSGLLKVLDIMPLTLHACMHQKQRLRNLEQFARLEDCVLLATDVAARGLDIPKVQHVIHYQVPRTSEIYVHRSGRTARATNEGLSLMLIGPEDVINFKKIYKTLKKDEDIPLFPVQTKYMDVVKERIRLARQIEKSEYRNFQACLHNSWIEQAAAALEIELEEDMYKGGKADQQEERRRQKQMKVLKKELRHLLSQPLFTESQKTKYPTQSGKPPLLVSAPSKSESALSCLSKQKKKKTKKPKEPQPEQPQPSTSAN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
别名 EC:3.6.4.13, DDX 24, ATP-dependent RNA helicase DDX24, 241145G10
计算分子量 96 kDa
观测分子量 120 kDa
GenBank蛋白编号BC008847
基因名称 DDX24
Gene ID (NCBI) 57062
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9GZR7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX24 is a family member of Asp-Glu-Ala-Asp (DEAD) box containing RNA helicases, and DEAD-box RNA helicases are characterized by a conserved DEAD motif. DDX24 was associated with cancer development, viral infection,and vascular malformation.

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