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验证数据展示

  • WB analysis using 80932-1-RR (same clone as 80932-1-PBS)

    WB analysis using 80932-1-RR (same clone as 80932-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80932-1-RR (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80932-1-RR (same clone as 80932-1-PBS)

    IF Staining of HepG2 using 80932-1-RR (same clone as 80932-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (80932-1-RR, Clone: 5E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80932-1-RR (same clone as 80932-1-PBS)

    IF Staining of HepG2 using 80932-1-RR (same clone as 80932-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (80932-1-RR, Clone: 5E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 80932-1-RR (same clone as 80932-1-PBS)

    WB analysis of HeLa using 80932-1-RR (same clone as 80932-1-PBS)

    WB result of DDX21 antibody (80932-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX21 transfected HeLa cells. This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80932-1-RR

    Affinity and Kinetic Characterization of 80932-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80932-1-RR against Human DDX21 were performed. The affinity constant is 77.7 nM.

  • Featured Product
  • KD/KO验证
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DDX21 Recombinant monoclonal antibody, PBS Only

DDX21 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 80932-1-PBS

产品说明书

CloneNo. 5E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

5E7, DEAD box protein 21, DExD-box helicase 21, EC:3.6.4.13, Gu alpha

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

80932-1-PBS targets DDX21 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0804

Product name: Recombinant human DDX21 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-180 aa of BC008071

Sequence: MPGKLRSDAGLESDTAMKKGETLRKQTEEKEKKEKPKSDKTEEIAEEEETVFPKAKQVKKKAEPSEVDMNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKKVVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPSEAASEESNSEIEQEIP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
别名 5E7, DEAD box protein 21, DExD-box helicase 21, EC:3.6.4.13, Gu alpha
计算分子量 87 kDa
观测分子量 87 kDa
GenBank蛋白编号BC008071
基因名称 DDX21
Gene ID (NCBI) 9188
RRIDAB_2918920
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NR30
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing. DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa.