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DDX19B Polyclonal antibody

DDX19B Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 18285-1-AP

Print datasheet

Synonyms

DBP5, DDX19, DDX19B, DEAD box protein 19B, DEAD box RNA helicase DEAD5, RNAh

验证数据展示

  • WB analysis of K-562 using 18285-1-AP

    WB analysis of K-562 using 18285-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 18285-1-AP

    IP experiment of K-562 using 18285-1-AP

    IP result of anti-DDX19B (IP:18285-1-AP, 4ug; Detection:18285-1-AP 1:500) with K-562 cells lysate 4000ug.

经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IP detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18285-1-AP targets DDX19B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DDX19B fusion protein Ag13165 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-As) box polypeptide 19B
Synonyms DBP5, DDX19, DDX19B, DEAD box protein 19B, DEAD box RNA helicase DEAD5, RNAh
Calculated Molecular Weight479aa,54 kDa; 370aa,42 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC010008
Gene Symbol DDX19B
Gene ID (NCBI) 11269
RRIDAB_10597088
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMR2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DDX19B antibody 18285-1-APDownload protocol
IP protocol for DDX19B antibody 18285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Acta Neuropathol Commun

Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease

Authors - Suman Rimal
View Article