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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83106-1-RR (same clone as 83106-1-PBS)

    WB analysis using 83106-1-RR (same clone as 83106-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83106-1-RR (DDX19A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83106-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83106-1-RR (same clone as 83106-1-PBS)

    FC experiment of U2OS using 83106-1-RR (same clone as 83106-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug DDX19A Recombinant antibody (83106-1-RR, Clone:230232B12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83106-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83106-1-RR

    Affinity and Kinetic Characterization of 83106-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83106-1-RR against Human DDX19A were performed. The affinity constant is below 1 pM.

DDX19A Recombinant antibody, PBS Only

DDX19A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83106-1-PBS

产品说明书

CloneNo. 230232B12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

EC:3.6.4.13, DDX19-like protein, DDX19L, DDX19 DDX19L, ATP-dependent RNA helicase DDX19A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83106-1-PBS targets DDX19A in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 DDX19A fusion protein Ag7362 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-As) box polypeptide 19A
别名 EC:3.6.4.13, DDX19-like protein, DDX19L, DDX19 DDX19L, ATP-dependent RNA helicase DDX19A
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC005162
基因名称 DDX19A
Gene ID (NCBI) 55308
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NUU7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX19A, also known as DDX19L, belongs to the DEAD-box helicase family. DDX19A was identified as a novel cytosolic RNA sensor that could activate the NLRP3 inflammasome during virus infection. In addition, DDX19A was proven to be associated with NADPH oxidase 1 (NOX1)-mediated oxidative stress in tumor necrosis factor (TNF)-α-induced A549 cell (PMID: 33968728). It may represent biomarkers of metastasis and novel therapeutic targets in CSCC patients.