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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83106-1-RR (same clone as 83106-1-PBS)

    WB analysis using 83106-1-RR (same clone as 83106-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83106-1-RR (DDX19A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83106-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83106-1-RR (same clone as 83106-1-PBS)

    FC experiment of U2OS using 83106-1-RR (same clone as 83106-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug DDX19A Recombinant antibody (83106-1-RR, Clone:230232B12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83106-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83106-1-RR

    Affinity and Kinetic Characterization of 83106-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83106-1-RR against Human DDX19A were performed. The affinity constant is below 1 pM.

    {{ptg:ProductFeaturesInfo}}

DDX19A Recombinant monoclonal antibody, PBS Only

DDX19A Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83106-1-PBS

产品说明书

CloneNo. 230232B12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

EC:3.6.4.13, DDX19-like protein, DDX19L, DDX19 DDX19L, ATP-dependent RNA helicase DDX19A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83106-1-PBS targets DDX19A in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7362

Product name: Recombinant human DDX19A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 124-478 aa of BC005162

Sequence: MMLAEPPQNLIAQSQSGTGKTAAFVLAMLSRVEPSDRYPQCLCLSPTYELALQTGKVIEQMGKFYPELKLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIATQGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNVIKLKREEETLDTIKQYYVLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMMVEQRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-As) box polypeptide 19A
别名 EC:3.6.4.13, DDX19-like protein, DDX19L, DDX19 DDX19L, ATP-dependent RNA helicase DDX19A
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC005162
基因名称 DDX19A
Gene ID (NCBI) 55308
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NUU7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX19A, also known as DDX19L, belongs to the DEAD-box helicase family. DDX19A was identified as a novel cytosolic RNA sensor that could activate the NLRP3 inflammasome during virus infection. In addition, DDX19A was proven to be associated with NADPH oxidase 1 (NOX1)-mediated oxidative stress in tumor necrosis factor (TNF)-α-induced A549 cell (PMID: 33968728). It may represent biomarkers of metastasis and novel therapeutic targets in CSCC patients.