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验证数据展示

  • WB analysis using 15209-1-AP

    WB analysis using 15209-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15209-1-AP (DDX19A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 15209-1-AP

    IHC staining of human ovary tumor using 15209-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15209-1-AP (DDX19A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 15209-1-AP

    IHC staining of human ovary tumor using 15209-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15209-1-AP (DDX19A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DDX19A Polyclonal antibody

DDX19A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15209-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

DDX19 DDX19L, DDX19 like protein, DDX19A, DDX19L, DEAD box protein 19A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, K-562 cells, HeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15209-1-AP targets DDX19A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 DDX19A fusion protein Ag7362 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-As) box polypeptide 19A
别名 DDX19 DDX19L, DDX19 like protein, DDX19A, DDX19L, DEAD box protein 19A
计算分子量 54 kDa
观测分子量44 kDa and 54 kDa
GenBank蛋白编号BC005162
基因名称 DDX19A
Gene ID (NCBI) 55308
RRIDAB_3085451
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUU7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX19A, also known as DDX19L, belongs to the DEAD-box helicase family. DDX19A was identified as a novel cytosolic RNA sensor that could activate the NLRP3 inflammasome during virus infection. In addition, DDX19A was proven to be associated with NADPH oxidase 1 (NOX1)-mediated oxidative stress in tumor necrosis factor (TNF)-α-induced A549 cell (PMID: 33968728). DDX19A has 2 isoforms with the molecular mass of 44 and 54 kDa. DDX19A may represent biomarkers of metastasis and novel therapeutic targets in CSCC patients.

实验方案

Product Specific Protocols
WB protocol for DDX19A antibody 15209-1-APDownload protocol
IHC protocol for DDX19A antibody 15209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol

DDX19A Senses Viral RNA and Mediates NLRP3-Dependent Inflammasome Activation.

Authors - Jiangnan Li
View Article