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验证数据展示

  • WB analysis using 85789-2-RR (same clone as 85789-2-PBS)

    WB analysis using 85789-2-RR (same clone as 85789-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85789-2-RR (DDX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85789-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02107-2

    Sandwich ELISA standard curve of MP02107-2

    Sandwich ELISA standard curve of MP02107-2, Human DDX18 Recombinant Matched Antibody Pair - PBS only. 85789-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27457. 85789-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP02107-2

    Sample test of MP02107-2

    The mean DDX18 concentration was determined to be 249.8 ng/mL in HEK-293T cell extract based on a 2.8 mg/mL extract load, 244.1 ng/mL in K-562 cell extract based on a 2.5 mg/mL extract load and 166.4 ng/mL in HepG2 cell extract based on a 1.8 mg/mL extract load.

  • IF Staining of HepG2 using 85789-2-RR (same clone as 85789-2-PBS)

    IF Staining of HepG2 using 85789-2-RR (same clone as 85789-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX18 antibody (85789-2-RR, Clone: 250053A4 ) at dilution of 1:2500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85789-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85789-2-RR (same clone as 85789-2-PBS)

    IF Staining of HeLa using 85789-2-RR (same clone as 85789-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX18 antibody (85789-2-RR, Clone: 250053A4 ) at dilution of 1:2500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85789-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02107-1

    Cytometric bead array standard curve of MP02107-1

    Cytometric bead array standard curve of MP02107-1, DDX18 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85789-2-PBS. Detection antibody: 85789-1-PBS. Standard: Ag27457. Range: 1.563-200 ng/mL.

DDX18 Recombinant antibody, PBS Only (Capture)

DDX18 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85789-2-PBS

产品说明书

CloneNo. 250053A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

ATP-dependent RNA helicase DDX18, cPERP-D, DEAD box protein 18, EC:3.6.4.13, MrDb

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85789-2-PBS targets DDX18 as part of a matched antibody pair:

MP02107-1: 85789-2-PBS capture and 85789-1-PBS detection (validated in Cytometric bead array)

MP02107-2: 85789-2-PBS capture and 85789-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 DDX18 fusion protein Ag27457 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
别名 ATP-dependent RNA helicase DDX18, cPERP-D, DEAD box protein 18, EC:3.6.4.13, MrDb
计算分子量 75 kDa
观测分子量80 kDa
GenBank蛋白编号BC001238
基因名称 DDX18
Gene ID (NCBI) 8886
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NVP1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX18 (DEAD-box helicase 18) is a protein belonging to the DEAD-box deconjugating enzyme family that is widely involved in RNA metabolism. It plays a key role in the assembly of the ribosomal small subunit (SSU) and ensures efficient intracellular protein synthesis. DDX18 unwinds RNA double strands through its deconjugating enzyme activity, which is essential for the proper processing and functional execution of RNA molecules. In addition, DDX18 is a multifunctional RNA deconjugating enzyme involved in a variety of signaling pathways, playing a key role not only in ribosome assembly and RNA metabolism, but also in the regulation of a variety of biological processes such as cell cycle, genome stability and stem cell pluripotency.