验证数据展示
经过测试的应用
| Positive WB detected in | SGC-7901 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85709-1-RR targets DDX10 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | DDX10 fusion protein Ag20766 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 |
| 别名 | DEAD box protein 10, EC:3.6.4.13, HRH J8, Probable ATP-dependent RNA helicase DDX10 |
| 计算分子量 | 875 aa, 101 kDa |
| 观测分子量 | 101-110 kDa |
| GenBank蛋白编号 | BC091521 |
| 基因名称 | DDX10 |
| Gene ID (NCBI) | 1662 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13206 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDX10, DEAD-box helicase 10, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Fusion of this gene and the nucleoporin gene NUP98 is found in leukemogenesis (PMID:20339440).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX10 antibody 85709-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |