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  • Featured Product
  • KD/KO Validated

DDX10 Polyclonal antibody

DDX10 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17857-1-AP

Print datasheet

Synonyms

DDX10, DEAD box protein 10, HRH J8

验证数据展示

  • WB analysis of SMMC-7721 using 17857-1-AP

    WB analysis of SMMC-7721 using 17857-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 17857-1-AP

    IHC staining of human kidney using 17857-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse testis using 17857-1-AP

    IHC staining of mouse testis using 17857-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 17857-1-AP

    IHC staining of mouse testis using 17857-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSMMC-7721 cells, HeLa cells
Positive IHC detected inmouse testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17857-1-AP targets DDX10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DDX10 fusion protein Ag12317 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Synonyms DDX10, DEAD box protein 10, HRH J8
Calculated Molecular Weight 875 aa, 101 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC091521
Gene Symbol DDX10
Gene ID (NCBI) 1662
RRIDAB_2878454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13206
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX10, DEAD-box helicase 10, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Fusion of this gene and the nucleoporin gene NUP98 is found in leukemogenesis (PMID:20339440).

实验方案

Product Specific Protocols
WB protocol for DDX10 antibody 17857-1-APDownload protocol
IHC protocol for DDX10 antibody 17857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cancer Cell Int

DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35.

Authors - Xin Zhou
  • KD Validated
View Article
humanWB

Biochem Biophys Res Commun

Epigenetic down-regulated DDX10 promotes cell proliferation through Akt/NF-κB pathway in ovarian cancer.

Authors - Muhuizi Gai
  • KD Validated
View Article