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- KD/KO Validated
DDRGK1 Polyclonal antibody
DDRGK1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
IF/ICC, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, PC-3 cells, mouse liver tissue, mouse lung tissue, rat liver tissue, rat lung tissue |
Positive IHC detected in | mouse brain tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21445-1-AP targets DDRGK1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IP, IF, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, goat, bovine |
Immunogen | DDRGK1 fusion protein Ag13742 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DDRGK domain containing 1 |
Synonyms | C20orf116, DDRGK domain containing 1, DDRGK1, UFBP1 |
Calculated Molecular Weight | 314 aa, 36 kDa |
Observed Molecular Weight | 36-46 kDa |
GenBank Accession Number | BC000643 |
Gene Symbol | DDRGK1 |
Gene ID (NCBI) | 65992 |
RRID | AB_2827383 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96HY6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for DDRGK1 antibody 21445-1-AP | Download protocol |
IHC protocol for DDRGK1 antibody 21445-1-AP | Download protocol |
IF protocol for DDRGK1 antibody 21445-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Cell A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.
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Nat Cell Biol UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination. | ||
Cell Res UFMylation of RPL26 links translocation-associated quality control to endoplasmic reticulum protein homeostasis. | ||
Adv Sci (Weinh) DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination
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Sci Adv Mechanistic insights into the roles of the UFM1 E3 ligase complex in ufmylation and ribosome-associated protein quality control
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Nat Commun A critical role of DDRGK1 in endoplasmic reticulum homoeostasis via regulation of IRE1α stability.
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