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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis of mouse ovary using 87486-1-RR (same clone as 87486-1-PBS)

    WB analysis of mouse ovary using 87486-1-RR (same clone as 87486-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 87486-1-RR (DDIT4L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87486-1-PBS in a different storage buffer formulation.

  • WB analysis using 87486-1-RR (same clone as 87486-1-PBS)

    WB analysis using 87486-1-RR (same clone as 87486-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87486-1-RR (DDIT4L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87486-1-PBS in a different storage buffer formulation.

  • Affinity of 87486-1-RR

    Affinity of 87486-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87486-1-RR against Human DDIT4L were performed. The affinity constant is 1.09 nM.

DDIT4L Recombinant monoclonal antibody, PBS Only

DDIT4L Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87486-1-PBS

产品说明书

CloneNo. 252832G4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

DNA damage-inducible transcript 4-like protein, HIF-1 responsive protein RTP801-like, REDD2, REDD-2, RTP801L

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87486-1-PBS targets DDIT4L in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag29616

Product name: Recombinant human DDIT4L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-99 aa of BC013592

Sequence: MVATGSLSSKNPASISELLDCGYHPESLLSDFDYWDYVVPEPNLNEVIFEESTCQNLVKMLENCLSKSKQTKLGCSKVLVPEKLTQRIAQDVLRLSSTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DNA-damage-inducible transcript 4-like
别名 DNA damage-inducible transcript 4-like protein, HIF-1 responsive protein RTP801-like, REDD2, REDD-2, RTP801L
计算分子量 193 aa, 22 kDa
观测分子量20 kDa
GenBank蛋白编号BC013592
基因名称 DDIT4L
Gene ID (NCBI) 115265
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96D03
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA-damage-inducible transcript 4-like protein(DDIT4L), encoded by the stress responsive gene REDD2, is a negative regulator of mTOR signaling, and expressed predominantly in skeletal muscle. It regulates the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Also, DDIT4L involves in oxidized low-density lipoprotein-induced macrophage death sensitivity.

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