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验证数据展示

  • WB analysis using 28490-1-AP

    WB analysis using 28490-1-AP

    Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 28490-1-AP (DDIT4L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 28490-1-AP

    IHC staining of mouse ovary using 28490-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 28490-1-AP (DDIT4L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse ovary using 28490-1-AP

    IHC staining of mouse ovary using 28490-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 28490-1-AP (DDIT4L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DDIT4L Polyclonal antibody

DDIT4L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28490-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

RTP801L, REDD-2, REDD2, HIF-1 responsive protein RTP801-like, DNA damage-inducible transcript 4-like protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse ovary tissue
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28490-1-AP targets DDIT4L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag29616

Product name: Recombinant human DDIT4L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-99 aa of BC013592

Sequence: MVATGSLSSKNPASISELLDCGYHPESLLSDFDYWDYVVPEPNLNEVIFEESTCQNLVKMLENCLSKSKQTKLGCSKVLVPEKLTQRIAQDVLRLSSTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DNA-damage-inducible transcript 4-like
别名 RTP801L, REDD-2, REDD2, HIF-1 responsive protein RTP801-like, DNA damage-inducible transcript 4-like protein
计算分子量 193 aa, 22 kDa
观测分子量22 kDa
GenBank蛋白编号BC013592
基因名称 DDIT4L
Gene ID (NCBI) 115265
RRIDAB_3669660
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96D03
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA-damage-inducible transcript 4-like protein(DDIT4L), encoded by the stress responsive gene REDD2, is a negative regulator of mTOR signaling, and expressed predominantly in skeletal muscle. It regulates the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Also, DDIT4L involves in oxidized low-density lipoprotein-induced macrophage death sensitivity.

实验方案

Product Specific Protocols
IHC protocol for DDIT4L antibody 28490-1-APDownload protocol
WB protocol for DDIT4L antibody 28490-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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