验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, U-937 cells, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
13968-1-AP targets DDI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, sheep |
| 免疫原 |
CatNo: Ag4992 Product name: Recombinant human DDI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-396 aa of BC022017 Sequence: MLITVYCVRRDLSEVTFSLQVSPDFELRNFKVLCEAESRVPVEEIQIIHMERLLIEDHCSLGSYGLKDGDIVVLLQKDNVGPRAPGRAPNQPRVDFSGIAVPGTSSSRPQHPGQQQQRTPAAQRSQGLASGEKVAGLQGLGSPALIRSMLLSNPHDLSLLKERNPPLAEALLSGSLETFSQVLMEQQREKALREQERLRLYTADPLDREAQAKIEEEIRQQNIEENMNIAIEEAPESFGQVTMLYINCKVNGHPLKAFVDSGAQMTIMSQACAERCNIMRLVDRRWAGVAKGVGTQRIIGRVHLAQIQIEGDFLQCSFSILEDQPMDMLLGLDMLRRHQCSIDLKKNVLVIGTTGTQTYFLPEGELPLCSRMVSGQDESSDKEITHSVMDSGRKEH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DDI1, DNA-damage inducible 1, homolog 1 (S. cerevisiae) |
| 别名 | DDI1, Protein DDI1 homolog 1 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 47 kDa, 56 kDa |
| GenBank蛋白编号 | BC022017 |
| 基因名称 | DDI1 |
| Gene ID (NCBI) | 414301 |
| RRID | AB_2089244 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WTU0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ddi1(DNA-damage inducible 1)appears to release substrate from an ubiquitination complex, making the substrate available for deubiquitination to facilitate proteasomal degradation. Ddi1 could be a potential selective target for lung cancer treatment. Ddi1 runs as a doublet of 54/56 kDa on acrylamide gels besides the caculated MW 44 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDI1 antibody 13968-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Mol Histol Silibinin ameliorates cisplatin-induced acute kidney injury via activating Nfe2l1-mediated antioxidative response to suppress the ROS/MAPK signaling pathway. | ||
Int J Mol Sci The Effects of DDI1 on Inducing Differentiation in Ovine Preadipocytes via Oar-miR-432 |
