DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Recombinant Antibody for IF, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (3)

Applications

IF, IP, ELISA and More (3)

Conjugate

Unconjugated

CloneNo.

4K14

Cat No : 80010-1-RR

Print datasheet

Synonyms

DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag, Flag tag



经过测试的应用

Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

The antibody 80010-1-RR is suggested for IP and IF.

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80010-1-RR targets DYKDDDDK tag in RIP, IP, IF, FC, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications IF, IP, ELISA
Cited Applications CoIP, FC, IF, IP, RIP
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, monkey
Immunogen DYKDDDDK tag fusion protein Ag2329 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DYKDDDDK tag
Synonyms DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag, Flag tag
Gene Symbol
Gene ID (NCBI)
RRIDAB_2882940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
IF protocol for DYKDDDDK tag antibody 80010-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Mol Cancer

A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis

Authors - Kaixuan Zeng

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang

PLoS Biol

CMPK2 is a host restriction factor that inhibits infection of multiple coronaviruses in a cell-intrinsic manner

Authors - Mingjun Zhu
CoIP

Autophagy

Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress

Authors - Jia-min Yan

Cancer Lett

N-myristoyltransferase-1 deficiency blocks myristoylation of LAMTOR1 and inhibits bladder cancer progression.

Authors - Yi Sun
humanCoIP

Cancer Lett

ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer

Authors - Chen Zhou