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验证数据展示

  • WB analysis using 66010-1-Ig (same clone as 66010-1-PBS)

    WB analysis using 66010-1-Ig (same clone as 66010-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 66010-1-Ig (same clone as 66010-1-PBS)

    IHC staining of human appendicitis using 66010-1-Ig (same clone as 66010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66010-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDB1 antibody included
  • Featured Product
  • KD/KO验证

DDB1 Monoclonal antibody, PBS Only

DDB1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66010-1-PBS

产品说明书

CloneNo. 4F6C6

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

4F6C6, Damage-specific DNA-binding protein 1, DDBA, DNA damage-binding protein 1, DNA damage-binding protein a

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DDB1 Ready-To-Use IHC Kit. DDB1 primary antibody included.

产品信息

66010-1-PBS targets DDB1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17903

Product name: Recombinant human DDB1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-400 aa of BC011686

Sequence: MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHA

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 damage-specific DNA binding protein 1, 127kDa
别名 4F6C6, Damage-specific DNA-binding protein 1, DDBA, DNA damage-binding protein 1, DNA damage-binding protein a
计算分子量 1140 aa, 127 kDa
观测分子量 127 kDa
GenBank蛋白编号BC011686
基因名称 DDB1
Gene ID (NCBI) 1642
RRIDAB_10950859
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16531
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.

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