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验证数据展示

  • WB analysis using 85849-4-RR

    WB analysis using 85849-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85849-4-RR (DCUN1D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85849-4-RR

    Affinity and Kinetic Characterization of 85849-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85849-4-RR against Human DCUN1D1 were performed. The affinity constant is 23.8 pM.

DCUN1D1 Recombinant antibody

DCUN1D1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85849-4-RR

产品说明书

CloneNo. 250196B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

DCN1, DCN1 like protein 1, DCN1-like protein 1, DCNL1, DCUN 1D1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HT-29 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85849-4-RR targets DCUN1D1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 DCUN1D1 fusion protein Ag1440 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae)
别名 DCN1, DCN1 like protein 1, DCN1-like protein 1, DCNL1, DCUN 1D1
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC009478
基因名称 DCUN1D1
Gene ID (NCBI) 54165
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96GG9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

It has been found that DCUN1D1, also known as squamous cell carcinoma-related oncogene, expression may play a role in development of brain metastasis in patients with Non-small cell lung carcinoma (NSCLC). DCUN1D1-positive tumor cells may have the ability to disrupt the blood-brain-barrier and colonize the brain.

实验方案

Product Specific Protocols
WB protocol for DCUN1D1 antibody 85849-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols