验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse heart tissue, Transfected HEK-293 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
16684-1-AP targets DCTPP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | DCTPP1 fusion protein Ag10015 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | dCTP pyrophosphatase 1 |
别名 | CDA03, dCTP pyrophosphatase 1, dCTPase 1, DCTPP1, Deoxycytidine triphosphatase 1, RS21C6, XTP3TPA |
计算分子量 | 19 kDa |
观测分子量 | 19 kDa, 40 kDa, 80 kDa |
GenBank蛋白编号 | BC001344 |
基因名称 | DCTPP1 |
Gene ID (NCBI) | 79077 |
RRID | AB_2878299 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9H773 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCTPP1, also named as XTP3TPA, CDAO3 and RS21C6, is a hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. It has a central role in the balance of dCTP and the metabolism of deoxycytidine analogues, thus contributing to the preservation of genome integrity. DCTPP1 has some forms such as monomer (~20 kDa), Dimer (~40 kDa) and Homotetramer (~80 kDa).
实验方案
Product Specific Protocols | |
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WB protocol for DCTPP1 antibody 16684-1-AP | Download protocol |
IHC protocol for DCTPP1 antibody 16684-1-AP | Download protocol |
IF protocol for DCTPP1 antibody 16684-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |