验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse lung tissue, mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue, human kidney tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:600-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16947-1-AP targets DCTN6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, monkey |
| 免疫原 |
CatNo: Ag10561 Product name: Recombinant human DCTN6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-190 aa of BC013175 Sequence: MAEKTQKSVKIAPGAVVCVESEIRGDVTIGPRTVIHPKARIIAEAGPIVIGEGNLIEEQALIINAYPDNITPDTEDPEPKPMIIGTNNVFEVGCYSQAMKMGDNNVIESKAYVGRNVILTSGCIIGACCNLNTFEVIPENTVIYGADCLRRVQTERPQPQTLQLDFLMKILPNYHHLKKTMKGSSTPVKN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dynactin 6 |
| 别名 | WS 3, Protein WS-3, Protein WS 3, Dynactin subunit p27, Dynactin subunit 6 |
| 计算分子量 | 190 aa, 21 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC013175 |
| 基因名称 | DCTN6 |
| Gene ID (NCBI) | 10671 |
| RRID | AB_2245654 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00399 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DCTN6 antibody 16947-1-AP | Download protocol |
| IP protocol for DCTN6 antibody 16947-1-AP | Download protocol |
| WB protocol for DCTN6 antibody 16947-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit.
| ||
Curr Biol An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation. | ||
Mol Biol Cell Mycalolide B dissociates dynactin and abolishes retrograde axonal transport of dense-core vesicles. | ||
Neurobiol Aging Dynactin 6 deficiency enhances aging-associated dystrophic neurite formation in mouse brains.
| ||
Cell Rep The Chlamydia effector Dre1 binds dynactin to reposition host organelles during infection |
