验证数据展示
经过测试的应用
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DCTN5 Ready-To-Use IHC Kit. DCTN5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10182-1-AP targets DCTN5 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat, Monkey |
| 免疫原 |
CatNo: Ag0234 Product name: Recombinant human DCTN5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-182 aa of BC004191 Sequence: ELGELLYNKSEYIETASGNKVSRQSVLCGSQNIVLNGKTIVMNDCIIRGDLANVRVGRHCVVKSRSVIRPPFKKFSKGVAFFPLHIGDHVFIEEDCVVNAAQIGSYVHVGKNCVIGRRCVLKDCCKILDNTVLPPETVVPPFTVFSGCPGLFSGELPECTQELMIDVTKSYYQKFLPLTQV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dynactin 5 (p25) |
| 别名 | DCTN5, dynactin 5 (p25), Dynactin subunit 5, Dynactin subunit p25, p25 |
| 计算分子量 | 20 kDa |
| GenBank蛋白编号 | BC004191 |
| 基因名称 | DCTN5 |
| Gene ID (NCBI) | 84516 |
| RRID | AB_2088344 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BTE1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DCTN5 antibody 10182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit. | ||
J Hazard Mater Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria. | ||
Curr Biol An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation. | ||
Sci Rep Autophagy defects and related genetic variations in renal cell carcinoma with eosinophilic cytoplasmic inclusions. |