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验证数据展示

  • WB analysis of human brain using 17098-1-AP

    WB analysis of human brain using 17098-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 17098-1-AP

    IP experiment of Jurkat using 17098-1-AP

    IP result of anti-DCTN4 (IP:17098-1-AP, 4ug; Detection:17098-1-AP 1:700) with Jurkat cells lysate 2800ug.

  • IHC staining of human stomach using 17098-1-AP

    IHC staining of human stomach using 17098-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17098-1-AP (DCTN4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

DCTN4 Polyclonal antibody

DCTN4 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 17098-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IP, ELISA

别名

Dynactin subunit p62, Dynactin subunit 4, Dyn4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, HepG2 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17098-1-AP targets DCTN4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, monkey
免疫原 DCTN4 fusion protein Ag10579 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynactin 4 (p62)
别名 Dynactin subunit p62, Dynactin subunit 4, Dyn4
计算分子量 460 aa, 52 kDa
观测分子量 62 kDa
GenBank蛋白编号BC026323
基因名称 DCTN4
Gene ID (NCBI) 51164
RRIDAB_2276944
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJW0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DCTN4 antibody 17098-1-APDownload protocol
IHC protocol for DCTN4 antibody 17098-1-APDownload protocol
IP protocol for DCTN4 antibody 17098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
MonkeyWB

Mol Biol Cell

Dynactin's pointed-end complex is a cargo-targeting module.

Authors - Ting-Yu Yeh
  • KD Validated
View Article
humanWB

Onco Targets Ther

Linc01094 Accelerates the Growth and Metastatic-Related Traits of Glioblastoma by Sponging miR-126-5p.

Authors - Xin Xing Li
View Article