验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, A375 cells, A431 cells, COS-7 cells, MCF-7 cells, rat skin tissue |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13095-1-AP targets DCT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, monkey |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3714 Product name: Recombinant human DCT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC028311 Sequence: MSPLWWGFLLSCLGCKILPGAQGQFPRVCMTVDSLVNKECCPRLGAESANVCGSQQGRGQCTEVRADTRPWSGPYILRNQDDRELWPRKFFHRTCKCTGNFAGYNCGDCKFGWTGPNCERKKPPVIRQNIHSLSPQEREQFLGALDLAKKRVHPDYVITTQHWLGLLGPNGTQPQFANCSVYDFFVWLHYYSVRDTLLGPGRPYRAIDFSHQGPAFVTWHRYHLLCLERDLQRLIGNESFALPYWNFATGRNECDVCTDQLFGAARPDDPTLISRNSRFSSWETVCDSLDDYNHLVTLCNGTYEGLLRRNQMGRNSMKLPTLKDIRDCLSLQKFDNPPFFQNSTFSFRNA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2) |
| 别名 | TRP2, TRP 2, L-dopachrome tautomerase, L-dopachrome Delta-isomerase, EC:5.3.3.12 |
| 计算分子量 | 519 aa, 59 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC028311 |
| 基因名称 | DCT |
| Gene ID (NCBI) | 1638 |
| RRID | AB_2090576 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P40126 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DCT antibody 13095-1-AP | Download protocol |
| IHC protocol for DCT antibody 13095-1-AP | Download protocol |
| IP protocol for DCT antibody 13095-1-AP | Download protocol |
| WB protocol for DCT antibody 13095-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol Neuropsin (OPN5) Mediates Local Light-Dependent Induction of Circadian Clock Genes and Circadian Photoentrainment in Exposed Murine Skin. | ||
Hum Mol Genet Transcriptome analysis and molecular signature of human retinal pigment epithelium. | ||
J Invest Dermatol miR-137 inhibits the invasion of melanoma cells through downregulation of multiple oncogenic target genes. | ||
J Cell Mol Med Kaempferol promotes melanogenesis and reduces oxidative stress in PIG1 normal human skin melanocytes | ||
Sci Rep Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage. | ||
Pharmaceutics Anti-Melanogenic Mechanism of Tetrahydrocurcumin and Enhancing Its Topical Delivery Efficacy Using a Lecithin-Based Nanoemulsion. |
