验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HeLa cells, human liver tissue, Jurkat cells, rat liver tissue, rat spleen tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
13863-1-AP targets DCP1B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4845 Product name: Recombinant human DCP1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 269-618 aa of BC043437 Sequence: QGVVRSLSYEEPRRHSPPIEKQLCPAIQKLMVRSADLHPLSELPENRPCENGSTHSAGEFFTGPVQPGSPHNIGTSRGVQNASRTQNLFEKLQSTPGAANKCDPSTPAPASSAALNRSRAPTSVTPVAPGKGLAQPPQAYFNGSLPPQTVGHQAHGREQSTLPRQTLAISGSQTGSSGVISPQELLKKLQIVQQEQQLHASNRPALAAKFPVLAQSSGTGKPLESWINKTPNTEQQTPLFQVISPQRIPATAAPSLLMSPMVFAQPTSVPPKERESGLLPVGGQEPPAAATSLLLPIQSPEPSVITSSPLTKLQLQEALLYLIQNDDNFLNIIYEAYLFSMTQAAMKKTM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DCP1 decapping enzyme homolog B (S. cerevisiae) |
| 别名 | mRNA-decapping enzyme 1B, hDcp1b, EC:3.6.1.62, DCP1 |
| 计算分子量 | 618 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC043437 |
| 基因名称 | DCP1B |
| Gene ID (NCBI) | 196513 |
| RRID | AB_2090411 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IZD4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCP1B belongs to DCP1 family. DCP1B may play a role in the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. It may remove the 7-methyl guanine cap structure mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. DCP1B has 2 isoforms with the molecular mass of 56 and 68 kDa. DCP1B is associated with DM-related atherogenesis (PMID:33195466).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DCP1B antibody 13863-1-AP | Download protocol |
| IHC protocol for DCP1B antibody 13863-1-AP | Download protocol |
| IP protocol for DCP1B antibody 13863-1-AP | Download protocol |
| WB protocol for DCP1B antibody 13863-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
