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验证数据展示

  • WB analysis using 83753-4-RR (same clone as 83753-4-PBS)

    WB analysis using 83753-4-RR (same clone as 83753-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83753-4-RR (Decorin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83753-4-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83753-4-RR (same clone as 83753-4-PBS)

    FC experiment of HepG2 using 83753-4-RR (same clone as 83753-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83753-4-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83753-4-RR (same clone as 83753-4-PBS)

    FC experiment of HEK-293 using 83753-4-RR (same clone as 83753-4-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83753-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83753-4-RR

    Affinity and Kinetic Characterization of 83753-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83753-4-RR against Human Decorin were performed. The affinity constant is below 1 pM.

Decorin Recombinant monoclonal antibody, PBS Only

Decorin Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83753-4-PBS

产品说明书

CloneNo. 240781E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

DCN, 240781E3, CSCD, DSPG2, PG S2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DCN Ready-To-Use IHC Kit. DCN primary antibody included.

产品信息

83753-4-PBS targets Decorin in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6275

Product name: Recombinant human DCN protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-359 aa of BC005322

Sequence: MKATIILLLLAQVSWAGPFQQRGLFDFMLEDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 decorin
别名 DCN, 240781E3, CSCD, DSPG2, PG S2
计算分子量 359 aa, 40 kDa
观测分子量70 kDa
GenBank蛋白编号BC005322
基因名称 Decorin
Gene ID (NCBI) 1634
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP07585
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.

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