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DCLRE1B Polyclonal antibody

DCLRE1B Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 13203-1-AP

Print datasheet

Synonyms

5 exonuclease Apollo, APOLLO, DCLRE1B, hSNM1B, SNM1 homolog B, SNM1B, SNMIB

验证数据展示

  • WB analysis of Jurkat using 13203-1-AP

    WB analysis of Jurkat using 13203-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13203-1-AP (DCLRE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 13203-1-AP

    IF Staining of HepG2 using 13203-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13203-1-AP (DCLRE1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13203-1-AP targets DCLRE1B in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DCLRE1B fusion protein Ag3945 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae)
Synonyms 5 exonuclease Apollo, APOLLO, DCLRE1B, hSNM1B, SNM1 homolog B, SNM1B, SNMIB
Calculated Molecular Weight 532 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC029687
Gene Symbol DCLRE1B
Gene ID (NCBI) 64858
RRIDAB_2292695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H816
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interstrand cross-links (ICLs) is an extremely cytotoxic form of DNA damage that covalently tethers the two strands of the double helix, which inhibits fundamental nuclear functions, including transcription and DNA replication [PMID:21701511]. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links [PMID:10848582]. Furthermore, DCLRE1B was identified as a TRF2-interacting protein and, has a role in telomere maintenance in association with the Shelterin complex, preventing non-homologous end-joining-mediated chromosome fusions protecting leading strand telomeres [PMID:20619712].

实验方案

Product Specific Protocols
WB protocol for DCLRE1B antibody 13203-1-APDownload protocol
IF protocol for DCLRE1B antibody 13203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development

Authors - Nazareno Bona
View Article