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验证数据展示

  • WB analysis using 25450-1-AP (same clone as 25450-1-PBS)

    WB analysis using 25450-1-AP (same clone as 25450-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25450-1-AP (DCLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25450-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 25450-1-AP (same clone as 25450-1-PBS)

    IP experiment of mouse brain using 25450-1-AP (same clone as 25450-1-PBS)

    IP result of anti-DCLK2 (IP:25450-1-AP, 4ug; Detection:25450-1-AP 1:3000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 25450-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 25450-1-AP (same clone as 25450-1-PBS)

    IF Staining of HepG2 using 25450-1-AP (same clone as 25450-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCLK2 antibody (25450-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 25450-1-PBS in a different storage buffer formulation.

DCLK2 Polyclonal antibody, PBS Only

DCLK2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 25450-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

DCAMKL2, CLICK-II, CLICK2, CLICK II, CL2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25450-1-PBS targets DCLK2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22142

Product name: Recombinant human DCLK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 6-71 aa of BC032726

Sequence: MLQVNVEARCTAGQILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNSTTTGVSVIMFDLTV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 doublecortin-like kinase 2
别名 DCAMKL2, CLICK-II, CLICK2, CLICK II, CL2
计算分子量 766 aa, 84 kDa
观测分子量 83 kDa
GenBank蛋白编号BC032726
基因名称 DCLK2
Gene ID (NCBI) 166614
RRIDAB_3085796
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N568
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCLK2 belongs to the protein kinase superfamily, contains two N-terminal doublecortin domains, a C-terminal serine/threonine protein kinase domain, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains. DCLK2 is expressed in the brain, heart and eyes (PMID: 18075264).

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