验证数据展示
经过测试的应用
| Positive WB detected in | U-251 cells, U-118 MG cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31631-1-AP targets DCHS2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag35080 Product name: Recombinant human DCHS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2500-2600 aa of NM_001358235 Sequence: IFTISPVLLLDTISTTQFLVEASDGGNPDLRALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIISGNSQNNFHV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dachsous 2 (Drosophila) |
| 别名 | Cadherin 27, Cadherin like protein CDHJ, Cadherin like protein VR8, CDH27, CDHJ |
| 计算分子量 | 370 kDa |
| 观测分子量 | ~75 kDa |
| GenBank蛋白编号 | NM_001358235 |
| 基因名称 | DCHS2 |
| Gene ID (NCBI) | 54798 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6V1P9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCHS2 is a calcium-dependent cell adhesion molecule belonging to the cadherin superfamily. It plays an important role in cell-cell adhesion, tissue morphogenesis, and signal transduction, especially in forming cell polarity and tissue structure during development.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DCHS2 antibody 31631-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
