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验证数据展示

  • WB analysis of mouse placenta using 26978-1-AP (same clone as 26978-1-PBS)

    WB analysis of mouse placenta using 26978-1-AP (same clone as 26978-1-PBS)

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 26978-1-AP (DCDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26978-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 26978-1-AP (same clone as 26978-1-PBS)

    WB analysis of HepG2 using 26978-1-AP (same clone as 26978-1-PBS)

    WB result of DCDC2 antibody (26978-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCDC2 transfected HepG2 cells. This data was developed using the same antibody clone with 26978-1-PBS in a different storage buffer formulation.

  • IHC staining of human Hepatocellular carcinoma using 26978-1-AP (same clone as 26978-1-PBS)

    IHC staining of human Hepatocellular carcinoma using 26978-1-AP (same clone as 26978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human Hepatocellular carcinoma slide using 26978-1-AP (DCDC2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26978-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DCDC2 antibody included
  • IF Staining of human liver cancer using 26978-1-AP (same clone as 26978-1-PBS)

    IF Staining of human liver cancer using 26978-1-AP (same clone as 26978-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver cancer tissue using DCDC2 antibody (26978-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26978-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DCDC2 Polyclonal antibody, PBS Only

DCDC2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 26978-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

DCDC2A, Doublecortin domain-containing protein 2, Protein RU2S, RU2, RU2S

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® DCDC2 Ready-To-Use IHC Kit. DCDC2 primary antibody included.

产品信息

26978-1-PBS targets DCDC2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25577

Product name: Recombinant human DCDC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 351-476 aa of BC050704

Sequence: EDGEKANKDAEQKEDFSGMNGDLEEEGGREATDAPEQVEEILDHSEQQARPARVNGGTDEENGEELQQVNNELQLVLDKERKSQGAGSGQDEADVDPQRPPRPEVKITSPEENENNQQNKDYAAVA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 doublecortin domain containing 2
别名 DCDC2A, Doublecortin domain-containing protein 2, Protein RU2S, RU2, RU2S
计算分子量 476 aa, 53 kDa
观测分子量 52 kDa
GenBank蛋白编号BC050704
基因名称 DCDC2
Gene ID (NCBI) 51473
RRIDAB_2880709
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHG0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCDC2 (Doublecortin domain-containing protein 2), also known as KIAA1154. DCDC2 is expressed in the central nervous system of adults and fetuses, especially in the olfactory cortex, inferior temporal cortex, medial temporal cortex, hypothalamus, amygdala and hippocampus. DCDCDC2 protein contains two dicortical protein domains that bind to microtubules and enhance microtubule polymerization, which may play a role in neuronal migration and affect the signal transduction of primary cilia. In addition, DCDC2 also plays a role in inhibiting classical WNT signaling. DCDC2 may affect the development and function of the brain by affecting the migration of neurons and the signal conduction of primary cilia, and then it is related to reading ability (PMID: 21457949). The molecular weight of DCDC2 is 52 kDa, it also has another isoform with a molecular weight of 25 kDa.

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