验证数据展示
经过测试的应用
| Positive WB detected in | MKN-45 cells, human liver tissue, human brain tissue, SW 1990 cells, A549 cells, U2OS cells |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
13168-1-AP targets DCBLD2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3821 Product name: Recombinant human DCBLD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 519-743 aa of BC029658 Sequence: YDLPYWDRAGFYLMVSLACRHNEGWWKGMKQFLPAKAVDHEETPVRYSSSEVNHLSPREVTTVLQADSAEYAQPLVGGIVGTLHQRSTFKPEEGKEAGYADLDPYNSPGQEVYHAYAEPLPITGPEYATPIIMDMSGHPTTSVGQPSTSTFKATGNQPPPLVGTYNTLLSRTDSCSSAQAQYDTPKAGKPGLPAPDELVYQVPQSTQEVSGAGRDGECDVFKEIL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | discoidin, CUB and LCCL domain containing 2 |
| 别名 | CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein, ESDN |
| 计算分子量 | 775 aa, 89 kDa |
| 观测分子量 | 93 kDa, 127 kDa |
| GenBank蛋白编号 | BC029658 |
| 基因名称 | DCBLD2 |
| Gene ID (NCBI) | 131566 |
| RRID | AB_2292662 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96PD2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCBLD2 (also known as CLCP1 or ESDN) is a transmembrane protein belonging to the DCBLD family, characterized by extracellular discoidin, CUB, and LCCL domains. It plays roles in cell adhesion, signaling, and vascular biology, with emerging implications in cancer progression, angiogenesis, and cardiovascular diseases. DCBLD2 is upregulated in certain tumors (e.g., glioblastoma, lung cancer) and may regulate receptor tyrosine kinase (RTK) pathways, including EGFR and PDGFR. Its exact mechanisms remain under investigation, but it is suggested to influence cell migration, proliferation, and tumor microenvironment interactions. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DCBLD2 antibody 13168-1-AP | Download protocol |
| IP protocol for DCBLD2 antibody 13168-1-AP | Download protocol |
| WB protocol for DCBLD2 antibody 13168-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Environ Toxicol Comprehensive analyses and experimental verification of NETs and an EMT gene signature for prognostic prediction, immunotherapy, and chemotherapy in pancreatic adenocarcinoma
| ||
Am J Cancer Res In silico development and in vitro validation of a novel five-gene signature for prognostic prediction in colon cancer | ||
Aging (Albany NY) Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin |
