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DCBLD2 Polyclonal antibody

DCBLD2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13168-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IP, ELISA

ESDN, Endothelial and smooth muscle cell-derived neuropilin-like protein, Discoidin, CUB and LCCL domain-containing protein 2, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, CLCP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inMKN-45 cells, human liver tissue, human brain tissue, SW 1990 cells, U2OS cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13168-1-AP targets DCBLD2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 DCBLD2 fusion protein Ag3821 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 discoidin, CUB and LCCL domain containing 2
别名 ESDN, Endothelial and smooth muscle cell-derived neuropilin-like protein, Discoidin, CUB and LCCL domain-containing protein 2, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, CLCP1
计算分子量 775 aa, 89 kDa
观测分子量93 kDa, 127 kDa
GenBank蛋白编号BC029658
基因名称 DCBLD2
Gene ID (NCBI) 131566
RRIDAB_2292662
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PD2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).

实验方案

Product Specific Protocols
WB protocol for DCBLD2 antibody 13168-1-APDownload protocol
IHC protocol for DCBLD2 antibody 13168-1-APDownload protocol
IP protocol for DCBLD2 antibody 13168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Environ Toxicol

Comprehensive analyses and experimental verification of NETs and an EMT gene signature for prognostic prediction, immunotherapy, and chemotherapy in pancreatic adenocarcinoma

Authors - Jing Zhang
  • KD Validated
humanWB,IHC

Am J Cancer Res

In silico development and in vitro validation of a novel five-gene signature for prognostic prediction in colon cancer

Authors - Qiankun Zhu
mouseWB

Aging (Albany NY)

Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin

Authors - Yuqi Xin
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