验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HEK-293T cells, HuH-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32592-1-AP targets DCAF16 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36770 Product name: Recombinant human C4orf30 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC101716 Sequence: MGPRNPSPDHLSESESEEEENISYLNESSGEEWDSSEEEDSMVPNLSPLESLAWQVKCLLKYSTTWKPLNPNSWLYHAKLLDPSTPVHILREIGLRLSHCSHCVPKLEPIPEWPPLASCGVPPFQKPLTSPSRLSRDHATLNGALQFATKQLSRTLSRATPIPEYLKQIPNSCVSGCCCGWLTKTVKETTRTEPINTTYSYTDFQKAVNKLLTASL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 4 open reading frame 30 |
| 别名 | DDB1- and CUL4-associated factor 16, C4orf30 |
| 计算分子量 | 216 aa, 24 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC101716 |
| 基因名称 | C4orf30 |
| Gene ID (NCBI) | 54876 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9NXF7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DCAF16 is a member of the DDB1-CUL4 associated factor (DCAF) family. It is a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serving as a crucial substrate recognition element. DCAF16 plays a central role in the ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DCAF16 antibody 32592-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
