验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse brain tissue, rat brain tissue, NCI-H1299 cells |
| Positive IHC detected in | human stomach cancer tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DBT Ready-To-Use IHC Kit. DBT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1500-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12451-1-AP targets DBT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3101 Product name: Recombinant human DBT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC016675 Sequence: MAAVRMLRTWSRNAGKLICVRYFQTCGNVHVLKPNYVCFFGYPSFKYSHPHHFLKTTAALRGQVVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQSDKASVTITSRYDGVIKKLYYNLDDIAYVGKPLVDIETEALKDSEEDVVETPAVSHDEHTHQEIKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILPPSPKVEIMPPPPKPKDMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHFGYCDEIDLTELVKLREELKPIAFARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKASHNIGIAMDTEQG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dihydrolipoamide branched chain transacylase E2 |
| 别名 | BCKDHE2, BCKAD-E2, BCKADE2, BCATE2, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis |
| 计算分子量 | 482 aa, 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC016675 |
| 基因名称 | DBT |
| Gene ID (NCBI) | 1629 |
| RRID | AB_2089636 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P11182 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DBT antibody 12451-1-AP | Download protocol |
| IHC protocol for DBT antibody 12451-1-AP | Download protocol |
| WB protocol for DBT antibody 12451-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Metab Branched-chain amino acid catabolism in muscle affects systemic BCAA levels but not insulin resistance | ||
J Nutr Pyruvate Kinase M2 Supports Muscle Progenitor Cell Proliferation but Is Dispensable for Skeletal Muscle Regeneration after Injury. | ||
Heliyon Hyperactivation of mTOR and AKT in a cardiac hypertrophy animal model of Friedreich ataxia | ||
Cell Metab Extra-cardiac BCAA catabolism lowers blood pressure and protects from heart failure | ||
Front Immunol Overexpression of DBT suppresses the aggressiveness of renal clear cell carcinoma and correlates with immune infiltration | ||
J Thromb Haemost Branched-chain amino acids promote thrombocytopoiesis by activating mTOR signaling |
