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BRINP1 Monoclonal antibody

BRINP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human, pig, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10E3

Cat No : 60336-1-Ig

Print datasheet

Synonyms

BRINP1, DBC1, DBCCR1, deleted in bladder cancer 1, FAM5A, Protein FAM5A

验证数据展示

  • WB analysis of fetal human brain using 60336-1-Ig

    WB analysis of fetal human brain using 60336-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60336-1-Ig (DBC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 60336-1-Ig

    IF Staining of MCF-7 using 60336-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 60336-1-Ig(DBC1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected infetal human brain tissue, mouse brain tissue, pig brain tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60336-1-Ig targets BRINP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, pig, mouse
Immunogen BRINP1 fusion protein Ag6588 种属同源性预测
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Full Name deleted in bladder cancer 1
Synonyms BRINP1, DBC1, DBCCR1, deleted in bladder cancer 1, FAM5A, Protein FAM5A
Calculated Molecular Weight 761 aa, 88 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberBC021560
Gene Symbol BRINP1
Gene ID (NCBI) 1620
RRIDAB_2881445
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60477
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRINP1, also known as DBC1 and FAM5A, inhibits cell proliferation by negative regulation of the G1/S transition. It mediates cell death which is not of the classical apoptotic type and regulates expression of components of the plasminogen pathway.

实验方案

Product Specific Protocols
WB protocol for BRINP1 antibody 60336-1-IgDownload protocol
IF protocol for BRINP1 antibody 60336-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols