验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22638-1-AP targets CCAR2 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag18374 Product name: Recombinant human DBC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 571-923 aa of BC018269 Sequence: EPEKEEAAKEEATKEEEAIKEEVVKEPKDEAQNEGPATESEAPLKEDGLLPKPLSSGGEEEEKPRGEASEDLCEMALDPELLLLRDDGEEEFAGAKLEDSEVRSVASNQSEMEFSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVTQNICQYRSLQYSRQEGLDGGLPEEVLFGNLDLLPPPGKSTKPGAAPTEHKALVSHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRVRLAEAEETARTAERQKSQLQRLLQELRRRLTPLQLEIQRVVEKADSWVEKEEPAPSN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA1967 |
| 别名 | cell cycle and apoptosis regulator 2, DBC1, DBC-1, KIAA1967, Cell cycle and apoptosis regulator protein 2 |
| 计算分子量 | 923 aa, 103 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC018269 |
| 基因名称 | CCAR2 |
| Gene ID (NCBI) | 57805 |
| RRID | AB_2879138 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N163 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCAR2 also known as DBC1 (Deleted in Breast Cancer 1), KIAA1967, NET35, and p30DBC, is a multifaceted nuclear protein that plays a critical role in various cellular processes, including transcriptional regulation, apoptosis (programmed cell death), DNA damage response, metabolism, and the maintenance of circadian rhythms. The protein is able to bind to RNA polymerase II complexes and exhibits enzyme inhibitor activity.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CCAR2 antibody 22638-1-AP | Download protocol |
| IHC protocol for CCAR2 antibody 22638-1-AP | Download protocol |
| IP protocol for CCAR2 antibody 22638-1-AP | Download protocol |
| WB protocol for CCAR2 antibody 22638-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''. | ||
Biochem Cell Biol Chromatin remodeling factor CECR2 forms tissue-specific complexes with CCAR2 and LUZP1. | ||
Elife Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression | ||
Br J Pharmacol AdipoRon promotes amyloid-β clearance through enhancing autophagy via nuclear GAPDH-induced sirtuin 1 activation in Alzheimer's disease | ||
Redox Biol SIRT1 activated by AROS sensitizes glioma cells to ferroptosis via induction of NAD+ depletion-dependent activation of ATF3 | ||
Free Radic Biol Med CircMETTL9 targets CCAR2 to induce neuronal oxidative stress and apoptosis via mitochondria-mediated pathways following traumatic brain injury |
