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验证数据展示

  • WB analysis using 31459-1-AP (same clone as 31459-1-PBS)

    WB analysis using 31459-1-AP (same clone as 31459-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31459-1-AP (DAB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

  • WB analysis using 31459-1-AP (same clone as 31459-1-PBS)

    WB analysis using 31459-1-AP (same clone as 31459-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31459-1-AP (DAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 31459-1-AP (same clone as 31459-1-PBS)

    IHC staining of rat brain using 31459-1-AP (same clone as 31459-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 31459-1-AP (same clone as 31459-1-PBS)

    IHC staining of mouse brain using 31459-1-AP (same clone as 31459-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 31459-1-AP (same clone as 31459-1-PBS)

    IHC staining of mouse cerebellum using 31459-1-AP (same clone as 31459-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 31459-1-AP (same clone as 31459-1-PBS)

    IHC staining of mouse cerebellum using 31459-1-AP (same clone as 31459-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31459-1-AP (DAB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 31459-1-AP (same clone as 31459-1-PBS)

    IF Staining of HepG2 using 31459-1-AP (same clone as 31459-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DAB1 antibody (31459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31459-1-PBS in a different storage buffer formulation.

DAB1 Polyclonal antibody, PBS Only

DAB1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31459-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Disabled homolog 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31459-1-PBS targets DAB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag35376

Product name: Recombinant human DAB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 132-280 aa of NM_021080

Sequence: KEGNHRFVAIKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVYQTILEEDVEDPVYQYIVFEAGHEPIRDPETEENIYQVPTSQKKEGVYDVPKSQPVSNGYSFEDFEERFAAATPNRNLPTDFDEIFEATKAVTQLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 disabled homolog 1 (Drosophila)
别名 Disabled homolog 1
计算分子量64 kDa
观测分子量45 kDa, 80 kDa
GenBank蛋白编号NM_021080
基因名称 DAB1
Gene ID (NCBI) 1600
RRIDAB_3742431
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO75553
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Disabled-1 (Dab1) is an adaptor protein that is essential for neuronal migration and maturation in response to the extracellular protein Reelin. DAB1 protein docks to the intracellular part of the Reelin very low density lipoprotein receptor and apoE receptor type 2 and becomes tyrosine-phosphorylated following binding of Reelin to cortical neurons. The DAB1 protein contains a 180-amino acid N-terminal protein interaction/phosphotyrosine-binding (PTB) 1 domain that docks to the short cytoplasmic tail of the very low density lipoprotein receptor or apoE receptor type 2 at the level of NPXY motifs, with a preference for unphosphorylated motifs. Dab1 polypeptide bands ranging from 36 to 120 kDa have been identified in mouse embryonic brain, the 80-kDa Dab1 being the predominant form (PMID: 24313315).

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