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验证数据展示

  • WB analysis using 67287-1-Ig (same clone as 67287-1-PBS)

    WB analysis using 67287-1-Ig (same clone as 67287-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67287-1-Ig (DAAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67287-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 67287-1-Ig (same clone as 67287-1-PBS)

    IHC staining of human skin cancer using 67287-1-Ig (same clone as 67287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67287-1-Ig (DAAM1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67287-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DAAM1 antibody included

DAAM1 Monoclonal antibody, PBS Only

DAAM1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67287-1-PBS

产品说明书

CloneNo. 3F11F11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

3F11F11, Disheveled-associated activator of morphogenesis 1, FLJ41657, KIAA0666

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® DAAM1 Ready-To-Use IHC Kit. DAAM1 primary antibody included.

产品信息

67287-1-PBS targets DAAM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6809

Product name: Recombinant human DAAM1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 715-1068 aa of BC064999

Sequence: KDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSGSEEVFRSGALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPSVLNLNEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQPGDKFVSVVSQFITVASFSFSDVEDLLAEAKDLFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVSEAKQENENMRKKKEEEERRARMEAQLKEQRERERKMRKAKENSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRITNQMTDSSRERPITKLNF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dishevelled associated activator of morphogenesis 1
别名 3F11F11, Disheveled-associated activator of morphogenesis 1, FLJ41657, KIAA0666
计算分子量 123 kDa
观测分子量 120 kDa
GenBank蛋白编号BC064999
基因名称 DAAM1
Gene ID (NCBI) 23002
RRIDAB_2882553
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4D1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.

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