验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19923-1-AP targets NSG1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag13888 Product name: Recombinant human D4S234E protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-185 aa of BC001745 Sequence: MVKLGNNFAEKGTKQPLLEDGFDTIPLMTPLDVNQLQFPPPDKVVVKTKTEYEPDRKKGKARPPQIAEFTVSITEGVTERFKVSVLVLFALAFLTCVVFLVVYKVYKYDRACPDGFVLKNTQCIPEGLESYYAEQDSSAREKFYTVINHYNLAKQSITRSVSPWMSVLSEEKLSEQETEAAEKSA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DNA segment on chromosome 4 (unique) 234 expressed sequence |
| 别名 | D4S234E, D4S234, NEEP21, Neuron-enriched endosomal protein of 21 kDa, Neuron-specific protein family member 1 |
| 计算分子量 | 185 aa, 21 kDa |
| 观测分子量 | 16-21 kDa |
| GenBank蛋白编号 | BC001745 |
| 基因名称 | D4S234E |
| Gene ID (NCBI) | 27065 |
| RRID | AB_3741973 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42857 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSG1, also known as Neuron-Enriched Endosomal Protein of 21 kDa (NEEP21) , is a protein encoded by the NSG1 gene located on human chromosome 4p16.3 . The Neuron-Specific Gene (NSG) family includes NSG1, NSG2, and NSG3. As their name indicates they are expressed almost exclusively in neurons, and localize to multiple subcellular organelles including the Golgi apparatus, early and late endosomes, and the plasma membrane. As a highly enriched endosomal protein within neuronal cells, NSG1 has been discovered to facilitate the process of epithelial-mesenchymal transition (EMT) in esophageal squamous cell carcinoma (ESCC).(PMID: 37872157,PMID: 41415429)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NSG1 antibody 19923-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
