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验证数据展示

  • WB analysis using 68627-1-Ig (same clone as 68627-1-PBS)

    WB analysis using 68627-1-Ig (same clone as 68627-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 68627-1-Ig (same clone as 68627-1-PBS)

    IHC staining of human colon using 68627-1-Ig (same clone as 68627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CK8 antibody included
  • IHC staining of human colon using 68627-1-Ig (same clone as 68627-1-PBS)

    IHC staining of human colon using 68627-1-Ig (same clone as 68627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CK8 antibody included
  • Cytometric bead array standard curve of MP51080-1

    Cytometric bead array standard curve of MP51080-1

    Cytometric bead array standard curve of MP51080-1, Cytokeratin 8 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68627-1-PBS. Detection antibody: 68627-2-PBS. Standard:Ag0488. Range: 0.098-100 ng/mL.

Cytokeratin 8 Monoclonal antibody, PBS Only (Capture)

Cytokeratin 8 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 68627-1-PBS

产品说明书

CloneNo. 1F2H5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

KRT8, 1F2H5, CARD2, CK 8, CK8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CK8 Ready-To-Use IHC Kit. CK8 primary antibody included.

产品信息

68627-1-PBS targets Cytokeratin 8 as part of a matched antibody pair:

MP51080-1: 68627-1-PBS capture and 68627-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0488

Product name: Recombinant human KRT8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-483 aa of BC000654

Sequence: MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 8
别名 KRT8, 1F2H5, CARD2, CK 8, CK8
计算分子量 54 kDa
观测分子量50-52 kDa
GenBank蛋白编号BC000654
基因名称 Cytokeratin 8
Gene ID (NCBI) 3856
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP05787
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

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