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验证数据展示

  • WB analysis using 68295-1-Ig (same clone as 68295-1-PBS)

    WB analysis using 68295-1-Ig (same clone as 68295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT5/6 antibody included
  • IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    IHC staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68295-1-Ig (Cytokeratin 5/6 antibody) at dilution of 1:2300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT5/6 antibody included
  • IF Staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    IF Staining of human cervical cancer using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68295-1-Ig (same clone as 68295-1-PBS)

    IF Staining of HeLa using 68295-1-Ig (same clone as 68295-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 5/6 antibody (68295-1-Ig, Clone: 1F9E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68295-1-PBS in a different storage buffer formulation.

Cytokeratin 5/6 Monoclonal antibody, PBS Only

Cytokeratin 5/6 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 68295-1-PBS

产品说明书

CloneNo. 1F9E1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

KRT6B, Cytokeratin 6B, CK-6B, CK6B, CK5/6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy KRT5/6 Ready-To-Use IHC Kit. KRT5/6 primary antibody included.

产品信息

68295-1-PBS targets Cytokeratin 5/6 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Cytokeratin 5/6 fusion protein Ag11288 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 6B
别名 KRT6B, Cytokeratin 6B, CK-6B, CK6B, CK5/6
计算分子量 564 aa, 60 kDa
观测分子量60 kDa
GenBank蛋白编号BC034535
基因名称 Cytokeratin 6B
Gene ID (NCBI) 3854
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP04259
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6.