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验证数据展示

  • IHC staining of human breast cancer using 33702-1-AP

    IHC staining of human breast cancer using 33702-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 33702-1-AP (Cytokeratin 24 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 33702-1-AP

    IHC staining of human breast cancer using 33702-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 33702-1-AP (Cytokeratin 24 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 33702-1-AP

    IF Staining of A431 using 33702-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 24 antibody (33702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of A431 using 33702-1-AP

    IF Staining of A431 using 33702-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 24 antibody (33702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Cytokeratin 24 Polyclonal antibody

Cytokeratin 24 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 33702-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

CK-24, Cytokeratin-24, K24, KA24, Keratin, type I cytoskeletal 24

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33702-1-AP targets Cytokeratin 24 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag40474

Product name: Recombinant human KRT24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 426-525 aa of BC111968

Sequence: AEYKQLLDIKTRLEVEIETYRRLLDGEGGGSSFAEFGGRNSGSVNMGSRDLVSGDSRSGSCSGQGRDSSKTRVTKTIVEELVDGKVVSSQVSSISEVKVK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 24
别名 CK-24, Cytokeratin-24, K24, KA24, Keratin, type I cytoskeletal 24
GenBank蛋白编号BC111968
基因名称 KRT24
Gene ID (NCBI) 192666
RRIDAB_3743055
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ2M2I5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytokeratin 24 (CK-24), also known as Keratin 24 (KRT24), is a relatively recently identified type I cytokeratin. It is encoded by the KRT24 gene located on human chromosome 17. KRT24 is strongly expressed in keratinocytes, colon, spleen, and placenta. Previous research has suggested that KRT24 potentially acts as a oncogene in the early stages of colorectal cancer.

实验方案

Product Specific Protocols
IF protocol for Cytokeratin 24 antibody 33702-1-APDownload protocol
IHC protocol for Cytokeratin 24 antibody 33702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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