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验证数据展示

  • WB analysis using 86250-1-RR (same clone as 86250-1-PBS)

    WB analysis using 86250-1-RR (same clone as 86250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)

    IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT19 antibody included
  • IF Staining of MCF-7 using 86250-1-RR (same clone as 86250-1-PBS)

    IF Staining of MCF-7 using 86250-1-RR (same clone as 86250-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cytokeratin 19 antibody (86250-1-RR, Clone: 250894B1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)

    IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT19 antibody included

Cytokeratin 19 Recombinant antibody, PBS Only

Cytokeratin 19 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86250-1-PBS

产品说明书

CloneNo. 250894B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CK 19, CK19, KRT19, CK-19, Cytokeratin,Keratin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.

产品信息

86250-1-PBS targets Cytokeratin 19 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 19
别名 CK 19, CK19, KRT19, CK-19, Cytokeratin,Keratin
计算分子量 44 kDa
观测分子量44 kDa
GenBank蛋白编号BC002539
基因名称 Cytokeratin 19
Gene ID (NCBI) 3880
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08727
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.