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验证数据展示

  • WB analysis of HaCaT using 86761-3-RR (same clone as 86761-3-PBS)

    WB analysis of HaCaT using 86761-3-RR (same clone as 86761-3-PBS)

    HaCaT cells were subjected to SDS PAGE followed by western blot with 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86761-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse skin using 86761-3-RR (same clone as 86761-3-PBS)

    IHC staining of mouse skin using 86761-3-RR (same clone as 86761-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86761-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT15 antibody included
  • IHC staining of mouse skin using 86761-3-RR (same clone as 86761-3-PBS)

    IHC staining of mouse skin using 86761-3-RR (same clone as 86761-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86761-3-RR (Cytokeratin 15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86761-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT15 antibody included

Cytokeratin 15 Recombinant monoclonal antibody, PBS Only

Cytokeratin 15 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86761-3-PBS

产品说明书

CloneNo. 251445H2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

KRT15, CK 15, CK15, CK-15, Cytokeratin,Keratin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy KRT15 Ready-To-Use IHC Kit. KRT15 primary antibody included.

产品信息

86761-3-PBS targets Cytokeratin 15 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 15
别名 KRT15, CK 15, CK15, CK-15, Cytokeratin,Keratin
计算分子量 49 kDa
观测分子量46-50 kDa
GenBank蛋白编号BC002641
基因名称 KRT15
Gene ID (NCBI) 3866
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP19012
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytokeratin 15 (CK15, encoded by KRT15) is a type I intermediate filament protein belonging to the keratin family. It is predominantly expressed in the basal and suprabasal layers of stratified epithelia, with particularly strong expression in the bulge region of the hair follicle, a well-characterized niche for epithelial stem cells. CK15 serves as a reliable marker for quiescent stem cells in various tissues, including the skin, mammary gland, and prostate, and its expression is often maintained during early stages of epithelial differentiation. In pathological contexts, CK15 is utilized as an immunohistochemical marker to distinguish basal cell carcinomas from other cutaneous neoplasms and to identify subsets of aggressive breast and prostate cancers. Its role in maintaining stem cell properties and tissue homeostasis makes it a valuable tool in both developmental biology and diagnostic pathology.

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