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验证数据展示

  • WB analysis of A431 using 60247-1-Ig (same clone as 60247-1-PBS)

    WB analysis of A431 using 60247-1-Ig (same clone as 60247-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 60247-1-Ig (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

    IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT15 antibody included
  • IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

    IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT15 antibody included
  • IF Staining of HeLa using 60247-1-Ig (same clone as 60247-1-PBS)

    IF Staining of HeLa using 60247-1-Ig (same clone as 60247-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (60247-1-Ig, Clone: 6B4F8 ) at dilution of 1:25 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50002-1

    Sandwich ELISA standard curve of MP50002-1

    Sandwich ELISA standard curve of MP50002-1, KRT15/Cytokeratin 15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60247-1-PBS. Detection antibody: HRP-conjugated 60247-2-PBS. Standard: Ag0185. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP50002-1

    Cytometric bead array standard curve of MP50002-1

    Cytometric bead array standard curve of MP50002-1, KRT15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60247-1-PBS. Detection antibody: 60247-2-PBS. Standard: Ag0185. Range: 1.563-100 ng/mL.

Cytokeratin 15 Monoclonal antibody, PBS Only (Capture)

Cytokeratin 15 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 60247-1-PBS

产品说明书

CloneNo. 6B4F8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

KRT15, 6B4F8, CK 15, CK15, CK-15

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy KRT15 Ready-To-Use IHC Kit. KRT15 primary antibody included.

产品信息

60247-1-PBS targets Cytokeratin 15 as part of a matched antibody pair:

MP50002-1: 60247-1-PBS capture and 60247-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0185

Product name: Recombinant human KRT15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-450 aa of BC002641

Sequence: AYLKKNHEEEMKEFSSQLAGQVNVEMDAAPGVDLTRVLAEMREQYEAMAEKNRRDVEAWFFSKTEELNKEVASNTEMIQTSKTEITDLRRTMQELEIELQSQLSMKAGLENSLAETECRYATQLQQIQGLIGGLEAQLSELRCEMEAQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMAGIGIREASSGGGGSSSNFHINVEESVDGQVVS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 keratin 15
别名 KRT15, 6B4F8, CK 15, CK15, CK-15
计算分子量 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC002641
基因名称 KRT15
Gene ID (NCBI) 3866
RRIDAB_2881369
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP19012
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

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